Molecular Detection of Ureaplasma Urealyticum in Pregnant Woman Suffering from Urinary Tract Infection
DOI:
https://doi.org/10.59992/IJSR.2024.v3n1p11الكلمات المفتاحية:
pregnancy، urinary tract infection، mycoplasma، Ureaplasma urelyticiumالملخص
Mycoplasma is a genus of bacteria that lack a cell wall, The present study was designed to identify the Ureaplasma urelyticium which causes the pregnant women suffering uti. The study included 200 samples of 150 pregnant women and 50 controls from the Bint Al-Huda teaching Hospital and Al-Haboubi teaching Hospital in the Thi-Qar provainc. The presence of the GPO gene in urine samples by using the PCR used as an indicator for mycoplasma colonization of urinary tract of pregnant, then the species was detected by DNA sequencing. in this study Regarding the GPO gene most of the cases were carry mycoplasmas and majority of the control groups expressing the positive results so that there was no significant statistical differences where (p value = 0.069). The present results recorded that the presence of about 94% homology between studied samples with Ureaplasma urelyticium sequences. Twenty-one, 12 and 6 genetic variations of 16SrRNA gene were identified in Mycoplasma hominis, Mycoplasma, ureaplasma urelyticium. Some variations were variably distributed in the majority of the studied samples. The study showed that the ureaplasma urelyticium.is an important cause of UTI in pregnant women in Iraqi population.
المراجع
1- Huffman, J. W. (2023, December 8). pregnancy. Encyclopedia Britannica. https://www.britannica.com/science/pregnancy.
2- Habak, P. J., & Griggs Jr, R. P. (2019). Urinary tract infection in pregnancy.
3- Giraldo, P. C., Araujo, E. D., Junior, J. E., Amaral, R. L. G. D., Passos, M. R., & Gonçalves, A. K. (2012). The prevalence of urogenital infections in pregnant women experiencing preterm and full-term labor. Infectious diseases in obstetrics and gynecology, 2012.
4- Delzell Jr, J. E., & Lefevre, M. L. (2000). Urinary tract infections during pregnancy. American family physician, 61(3), 713-720.
5- Johansen, T. E. B., Botto, H., Cek, M., Grabe, M., Tenke, P., Wagenlehner, F. M., & Naber, K. G. (2011). Critical review of current definitions of urinary tract infections and proposal of an EAU/ESIU classification system. International journal of antimicrobial agents, 38, 64-70.
6- Stamm, W. E., & Norrby, S. R. (2001). Urinary tract infections: disease panorama and challenges. The Journal of infectious diseases, 183(Supplement_1), S1-S4.
7- Willey, J. M., Sherwood, L. M., & Woolverton, C. J. (2014). Prescott's microbiology. McGraw-Hill.
8- Osborn, C. O. (2018a). Everything you should know about ureaplasma. Retrieved from https://www.healthline.com/health/ureaplasma.
9- Couldwell, D. L., Tagg, K. A., Jeoffreys, N. J., & Gilbert, G. L. (2013). Failure of moxifloxacin treatment in Mycoplasma genitalium infections due to macrolide and fluoroquinolone resistance. International journal of STD & AIDS, 24(10), 822-828.
10- Ferreira, G., Blasina, F., Rey, M. R., Anesetti, G., Sapiro, R., Chavarría, L., ... & Nicolson, G. L. (2022). Pathophysiological and molecular considerations of viral and bacterial infections during maternal-fetal and–neonatal interactions of SARS-CoV-2, Zika, and Mycoplasma infectious diseases. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1868(1), 166285.
11- Johansen, T. E. B., Botto, H., Cek, M., Grabe, M., Tenke, P., Wagenlehner, F. M., & Naber, K. G. (2011). Critical review of current definitions of urinary tract infections and proposal of an EAU/ESIU classification system. International journal of antimicrobial agents, 38, 64-70.
12- Van Kuppeveld, F. J., Van Der Logt, J. T., Angulo, A. F., Van Zoest, M. J., Quint, W. G., Niesters, H. G., ... & Melchers, W. J. (1993). Genus-and species-specific identification of mycoplasmas by 16S rRNA amplification. Applied and environmental microbiology, 59(2), 655.
13- Tang YW, Stratton CW, Podzorski RP, Loeffelholz M, Hayden RT. Detection and characterization of molecular amplification products: agarose gel electrophoresis, southern blot hybridization, restriction enzyme digest analysis, and enzyme-linked immunoassay. Advanced Techniques in Diagnostic Microbiology. 2006:243-63.
14- Zhang, Z., Schwartz, S., Wagner, L., & Miller, W. (2000). A greedy algorithm for aligning DNA sequences. Journal of Computational biology, 7(1-2), 203-214.
15- Letunic, I., & Bork, P. (2019). Interactive Tree of Life (iTOL) v4: recent updates and new developments. Nucleic acids research, 47(W1), W256-W259.
16- Tamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: molecular evolutionary genetics analysis version 11. Molecular biology and evolution, 38(7), 3022-3027.
17- Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution, 4(4), 406-425.
18- Kumar S, Tamura K, Nei M. 2004. MEGA3: integrated software forMolecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform. 5(2):150–163.
19- Bayraktar, M. R., Ozerol, I. H., Gucluer, N., & Celik, O. (2010). Prevalence and antibiotic susceptibility of Mycoplasma hominis and Ureaplasma urealyticum in pregnant women. International Journal of Infectious Diseases, 14(2), e90-e95.
20- Clarridge III, J. E. (2004). Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clinical microbiology reviews, 17(4), 840-862.
21- Woese, C. R. (1987). Bacterial evolution. Microbiological Reviews, 51(2), 221–271. doi:10.1128/mr.51.2.221-271.1987.
22- Al-Daghistani, H. I., & Abdel-Dayem, M. (2010). Clinical significance of asymptomatic urogenital Mycoplasma hominis and Ureaplasma urealyticum in relation to seminal fluid parameters among infertile Jordanian males. Middle East Fertility Society Journal, 15(1), 29-34.
23- Latthe, P. M., Toozs-Hobson, P., & Gray, J. (2008). Mycoplasma and ureaplasma colonisation in women with lower urinary tract symptoms. Journal of obstetrics and gynaecology, 28(5), 519-521.
24- Musatovova, O., & Baseman, J. B. (2009). Analysis identifying common and distinct sequences among Texas clinical strains of Mycoplasma genitalium. Journal of clinical microbiology, 47(5), 1469-1475.
25- Safari, M., Bakhshi, A., Erami, M., Kheirkhah, B., Pourbakhsh, A., & Pourbabei, H. (2015). Sequences of Mycoplasma hominis in patients with urinary tract infection in a Hospital in Kashan, Iran. Research Journal of Microbiology, 10(6), 260.
26- Mohseni Moghadam N, Kheirkhah B, Mirshekari TR, Fasihi Harandi M, Tafsiri E. Isolation and molecular identification of mycoplasma genitalium from the secretion of genital tract in infertile male and female. Iran J Reprod Med. 2014 Sep;12(9):601-8. PMID: 25469132; PMCID: PMC4248144.
27- Bahaabadi, S. J., Moghadam, N. M., Kheirkhah, B., Farsinejad, A., & Habibzadeh, V. (2014). Isolation and molecular identification of Mycoplasma hominis in infertile female and male reproductive system. Nephro-Urology Monthly, 6(6).
